genotyping of chlamydia trachomatis from endocervical specimens in shiraz, iran

Genotyping of Chlamydia trachomatis from endocervical specimens in Shiraz, Iran

The objective of this study was to determine the prevalence and distribution of Chlamydia trachomatisgenotypes in Shiraz, Iran. Two hundred twelve cervical swab samples were collected from women attendingShahid Motahari Polyclinic in Shiraz, Iran. The endocervical specimens were screened for C. trachomatis byplasmid PCR. Genotyping was performed in C. trachomatis-positive samples by nested PCR ...

Genotyping of Chlamydia trachomatis from endocervical specimens of infertile Mexican women.

INTRODUCTION It has been reported in several countries that Chlamydia trachomatis genotypes D, E, and F are the ones more frequently associated with urogenital infections. In Mexico, the prevalence of serovars and genotypes is unknown. MATERIAL AND METHODS One hundred and fifty-two endocervical swabs were collected from infertile women to test for C. trachomatis. The PCR-based RFLP and automa...

Genotyping of Endocervical Chlamydia trachomatis Strains and Detection of Serological Markers of Acute and Chronic Inflammation in Their Host

Comparison of IDEIA III and cell culture for the detection of Chlamydia trachomatis in endocervical specimens.

A total of 803 endocervical samples were obtained from females with clinical or epidemiological histories suggesting chlamydia infection. These specimens were tested by IDEIA III and cell culture for the presence of Chlamydia trachomatis. After resolution of discrepant results by direct fluorescent-antibody staining of pelleted cell culture transport materials, IDEIA III demonstrated sensitivit...

Evaluation of a nonisotopic probe for detection of Chlamydia trachomatis in endocervical specimens.

A nonisotopic probe (Gen-Probe PACE; Gen-Probe, Inc., San Diego, Calif.) for detection of Chlamydia trachomatis in endocervical specimens was evaluated in 344 women attending a dysplasia clinic or an obstetrics clinic and 158 women who visited an emergency room. For each patient, the probe, a tissue cell culture, and a direct immunofluorescent-antibody test (DFA; MicroTrak; Syva Co., Palo Alto,...

The Prevalence of Endocervical Chlamydia trachomatis Infection Among Young Females in Kashan, Iran

BACKGROUND Chlamydia trachomatis is one of the major bacterial agents of the sexually transmitted diseases worldwide, especially among young females. There is no data regarding the prevalence of genital Chlamydia infection among young females in Kashan, Iran. OBJECTIVES The current study aimed to determine the prevalence of endocervical C. trachomatis infection among females aged 17 - 35 year...